EASDEC European Association for the Study of Diabetic Eye Complications
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EASDEC European Association for the Study of Diabetic Eye Complications |
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Abstracts: PagetClarisse Paget, Marc Lecomte, Daniel Ruggiero, Nicolas Wiernsperger and Michel Lagarde. Diabetic Microangiopathy Research Unit, LIPHA-INSERM U352, INSA-Lyon, 69621 Villeurbanne cedex, France. INDUCTION OF APOPTOSIS AND CATALASE ACTIVITY BY ADVANCED GLYCATION END PRODUCTS IN BOVINE RETINAL PERICYTES Advanced glycation end products (AGE) are thought to be involved in the pathogenesis of diabetic retinopathy. In the present study, we investigated whether AGE could induce apoptosis in bovine retinal pericytes (BRP) in culture. We further compared the effects of AGE and a cell permeable C2-ceramide on catalase activity. Apoptosis was detected morphologically by acridine orange/ethidium bromide staining and annexin-V labeling. Both techniques revealed a 5- to 8-fold increase in apoptosis rate (p<0.05) in BRP cultured with 20 µM BSA-AGE during 2 passages as compared to BSA-control. Incubation of pericytes with 32 µM of C2-ceramide for 15 hours induced a small but significant apoptosis rate in cells and increased catalase activity by 35.3 ± 4.6% (p<0.05). This rise reproduced the catalase activity increase observed in BRP cultured with BSA-AGE vs. BSA-control (+ 38.8 ± 6.2%, p<0.05). These results suggest that apoptosis induced by AGE or ceramide in pericytes could be linked to an intracellular free radical production and that apoptosis induced by AGE could perhaps explain the pericyte drop-out observed during diabetic retinopathy. |
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